They've already said that the Sony Spider-Mans are off-limits. So that includes:
Spider-Man movies (Tobey Maguire)
Amazing Spider-Man movies (Andrew Garfield)
Spider-Man: The New Animated Series (MTV CGI cartoon series)
The Spectacular Spider-Man (cartoon series)
Spider-Boy, the Amalgam character, has already been confirmed off-limits for obvious reasons.
I think it's safe to say that Lego Spider-Man is off-limits too.
Maybe the "Turn Off the Dark" musical Spider-Man is off-limits? That's just a guess though.
The other animated Spider-Mans are fine. Spider-Man Unlimited and Amazing Friends have already appeared, and 60s Spider-Man and Ultimate Spider-Man have already been confirmed. The Electric Company Spider-Man was confirmed to be appearing at the C2E2 panel.
I would love to see the Spider-man from Twisted Toyfare. That guy cracks me up.