Eh, maybe, maybe not. While the event is promoted as "Every Spider-Man and universe colliding into 616!!!111!!111!!!!", it is really alternate realities of alternate realities of Spider-Man colliding into 616, with only a few of them seemingly being the original realities.
Ultimate Marvel's designation is 1610 but the Ultimate we see in ASM 9 is designated 610. Hell, even the curent Spider-Man 2099 is like the 3rd or fourth that has been in 616 at some point or another. The "original" from back in the original series, another that was time traveling with...uh...Excalibur, I think, and the current guy.