Previous Post Post-scriptum: I didn't take Marvel Team-Up #1-9 into consideration and, thus, didn't include 2 appearances of Sandman and 2 of Morbius in my counting. Otherwise, Sandman would remain in the 9th spot whereas Morbius would move up to the 10th spot (ranking above the Enforcers).

This time, I took into account their appearances in other comics.

(1) Sandman
4+0=4
+23=27

(2) Green Goblin / Norman Osborn
9+11+1=21

(3) Doctor Octopus
8+11=19

(4) Lizard / Curt Connors
3+11=14

(5) Kingpin (& Schemer)
0+12=12
+2=14

(6) Kraven the Hunter (& Gog)
4+7=11
+3=14

(7) Chameleon
2+1=3
+6=9

(8) Rhino
0+3=3
+6=9

(9) Beetle
1+1=2
+7=9

(10) Vulture(s)
4+(1+1+2)=8

(11) Scorpion / Mac Gargan
3+0=3
+(4+1)=8

(12) Electro
2+1=3
+5=8

(13) Mysterio
3+3=6

(14) Spencer Smythe & Spider-Slayers
2+4=6

(15) Ox (Enforcers)
3+0=3
+3=6

(16) Morbius
0+2=2
+2=4

(17) Silvermane (& Man Mountain Marko, Big C)
0+3=3

(18) Hammerhead
0+3=3

(19) Molten Man
2+0=2

(20) Shocker
0+2=2